Detailed information of TA system TA025516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 763012..763645
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQD6
• Locus tag: MCAN_RS03405
• Protein ID: WP_003403365.1
• Coordinates: 763012..763395 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MCAN_RS03410
• Protein ID: WP_015292353.1
• Coordinates: 763424..763645 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS03380	758304..758840	+	537	WP_003403341.1	50S ribosomal protein L10	-
MCAN_RS03385	758877..759269	+	393	WP_003403353.1	50S ribosomal protein L7/L12	-
MCAN_RS03390	759262..759957	-	696	WP_014000338.1	TetR/AcrR family transcriptional regulator	-
MCAN_RS03395	760028..761533	+	1506	WP_014000339.1	carotenoid cleavage oxygenase	-
MCAN_RS03400	761614..762624	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
MCAN_RS03405	763012..763395	-	384	WP_003403365.1	ribonuclease VapC6	Toxin
MCAN_RS03410	763424..763645	-	222	WP_015292353.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MCAN_RS03415	763721..764437	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
MCAN_RS03420	764713..765021	-	309	WP_014000341.1	type II toxin-antitoxin system PemK/MazF family toxin	-
MCAN_RS03425	765008..765253	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	-
MCAN_RS03430	765363..765798	-	436	Protein_684	type II toxin-antitoxin system VapC family toxin	-
MCAN_RS03435	765795..766049	-	255	WP_162139305.1	antitoxin	-
MCAN_RS03440	766163..768526	+	2364	WP_014000343.1	arylsulfatase AtsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-44)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13959.73 Da
• Isoelectric Point: 6.0803

>T25516 WP_003403365.1 NC_015848:c763395-763012 [Mycobacterium canettii CIPT 140010059]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRWIDSAQHRLARAGAVG
ALSVVDLLICDTAAARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Nucleotide

• Length: 384 bp

>T25516 NC_015848:c763395-763012 [Mycobacterium canettii CIPT 140010059]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8346.49 Da
• Isoelectric Point: 5.8506

>AT25516 WP_015292353.1 NC_015848:c763645-763424 [Mycobacterium canettii CIPT 140010059]
VSVTQIDLDDEALAEVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSREQAKGWDYKGWVAARDDEKALGI

Nucleotide

• Length: 222 bp

>AT25516 NC_015848:c763645-763424 [Mycobacterium canettii CIPT 140010059]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGAGGTGATGCGCATCGCCGCAGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGCAGGCGA
AAGGCTGGGACTACAAGGGCTGGGTGGCCGCGCGGGACGACGAGAAGGCCCTGGGGATTTGA

Structures

Toxin

• AlphaFold DB: G0TQD6