Detailed information of TA system TA025514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 715006..715649
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ92
• Locus tag: MCAN_RS03155
• Protein ID: WP_014000322.1
• Coordinates: 715248..715649 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ91
• Locus tag: MCAN_RS03150
• Protein ID: WP_003403184.1
• Coordinates: 715006..715251 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS03120	710364..710621	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
MCAN_RS03125	710804..712039	-	1236	WP_014000321.1	ATP-binding protein	-
MCAN_RS03130	712290..712703	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	-
MCAN_RS03135	712700..712936	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
MCAN_RS03140	713041..713547	-	507	WP_003900973.1	two component system sensor kinase HK1	-
MCAN_RS20975	713661..713774	-	114	Protein_627	histidine kinase	-
MCAN_RS03145	714491..714961	+	471	WP_003898523.1	hypothetical protein	-
MCAN_RS03150	715006..715251	+	246	WP_003403184.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MCAN_RS03155	715248..715649	+	402	WP_014000322.1	type II toxin-antitoxin system VapC family toxin	Toxin
MCAN_RS22285	716037..716204	-	168	WP_077385191.1	DUF3800 domain-containing protein	-
MCAN_RS22290	716237..716434	-	198	WP_003403191.1	hypothetical protein	-
MCAN_RS03165	716514..717671	-	1158	WP_014000323.1	hypothetical protein	-
MCAN_RS03170	717723..717944	-	222	WP_003898525.1	DUF4926 domain-containing protein	-
MCAN_RS03175	718086..718691	+	606	WP_003898526.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-80)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14485.21 Da
• Isoelectric Point: 4.4463

>T25514 WP_014000322.1 NC_015848:715248-715649 [Mycobacterium canettii CIPT 140010059]
VIVDTSAIIAILRDEDDAAAYADALANADVRRLSAASYLECGIVLDSQRDPVISRALDELVEEAEFIVEPVTERQARLAR
AAFADFGRGSGHPAGLNFGDCLSYALAIDRREPLLWKGNDFGHTGVQRALDRR

Nucleotide

• Length: 402 bp

>T25514 NC_015848:715248-715649 [Mycobacterium canettii CIPT 140010059]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTGTCGAAGAAGCCGAGTTCATCGTCGAGCCGGTGACCGAGCGCCAGGCCCGGCTGGCCCGA
GCGGCGTTCGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCGCTGGC
GATCGATCGACGCGAGCCGCTGCTGTGGAAGGGCAATGATTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8813.12 Da
• Isoelectric Point: 10.3486

>AT25514 WP_003403184.1 NC_015848:715006-715251 [Mycobacterium canettii CIPT 140010059]
MALNIKDPSVHQAVKQIAKITGESQARAVATAVNERLARLRSDDLAARLLAIGHKTASRMSPEAKRLDHDALLYDERGLP
A

Nucleotide

• Length: 246 bp

>AT25514 NC_015848:715006-715251 [Mycobacterium canettii CIPT 140010059]
ATGGCGTTGAACATCAAAGATCCGTCGGTTCACCAGGCGGTCAAGCAGATCGCGAAAATCACCGGCGAATCTCAGGCTCG
GGCGGTGGCGACCGCGGTGAACGAGCGTCTGGCCAGACTGCGCAGCGACGATCTCGCCGCCCGGCTCTTGGCTATCGGCC
ACAAGACCGCGAGCAGGATGAGCCCGGAAGCAAAGCGCCTCGACCACGATGCTCTGCTGTATGACGAGCGAGGGCTGCCG
GCGTGA

Structures

Toxin

• AlphaFold DB: G0TQ92

Antitoxin

• AlphaFold DB: G0TQ91