Detailed information of TA system TA025513

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 712290..712936
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: MCAN_RS03130
• Protein ID: WP_003403137.1
• Coordinates: 712290..712703 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: MCAN_RS03135
• Protein ID: WP_003403139.1
• Coordinates: 712700..712936 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS03110	708373..709923	+	1551	WP_014000319.1	MCE family protein	-
MCAN_RS03115	709975..710367	-	393	WP_014000320.1	type II toxin-antitoxin system toxin ribonuclease C4	-
MCAN_RS03120	710364..710621	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
MCAN_RS03125	710804..712039	-	1236	WP_014000321.1	ATP-binding protein	-
MCAN_RS03130	712290..712703	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
MCAN_RS03135	712700..712936	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
MCAN_RS03140	713041..713547	-	507	WP_003900973.1	two component system sensor kinase HK1	-
MCAN_RS20975	713661..713774	-	114	Protein_627	histidine kinase	-
MCAN_RS03145	714491..714961	+	471	WP_003898523.1	hypothetical protein	-
MCAN_RS03150	715006..715251	+	246	WP_003403184.1	type II toxin-antitoxin system VapB family antitoxin	-
MCAN_RS03155	715248..715649	+	402	WP_014000322.1	type II toxin-antitoxin system VapC family toxin	-
MCAN_RS22285	716037..716204	-	168	WP_077385191.1	DUF3800 domain-containing protein	-
MCAN_RS22290	716237..716434	-	198	WP_003403191.1	hypothetical protein	-
MCAN_RS03165	716514..717671	-	1158	WP_014000323.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T25513 WP_003403137.1 NC_015848:c712703-712290 [Mycobacterium canettii CIPT 140010059]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T25513 NC_015848:c712703-712290 [Mycobacterium canettii CIPT 140010059]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT25513 WP_003403139.1 NC_015848:c712936-712700 [Mycobacterium canettii CIPT 140010059]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT25513 NC_015848:c712936-712700 [Mycobacterium canettii CIPT 140010059]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89