Detailed information of TA system TA025512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-PHD
• Location: 709975..710621
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ85
• Locus tag: MCAN_RS03115
• Protein ID: WP_014000320.1
• Coordinates: 709975..710367 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ86
• Locus tag: MCAN_RS03120
• Protein ID: WP_003403125.1
• Coordinates: 710364..710621 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS03100	705583..707109	+	1527	WP_003403112.1	virulence factor Mce family protein	-
MCAN_RS03105	707226..708368	+	1143	WP_044080049.1	virulence factor Mce family protein	-
MCAN_RS03110	708373..709923	+	1551	WP_014000319.1	MCE family protein	-
MCAN_RS03115	709975..710367	-	393	WP_014000320.1	type II toxin-antitoxin system toxin ribonuclease C4	Toxin
MCAN_RS03120	710364..710621	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	Antitoxin
MCAN_RS03125	710804..712039	-	1236	WP_014000321.1	ATP-binding protein	-
MCAN_RS03130	712290..712703	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	-
MCAN_RS03135	712700..712936	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
MCAN_RS03140	713041..713547	-	507	WP_003900973.1	two component system sensor kinase HK1	-
MCAN_RS20975	713661..713774	-	114	Protein_627	histidine kinase	-
MCAN_RS03145	714491..714961	+	471	WP_003898523.1	hypothetical protein	-
MCAN_RS03150	715006..715251	+	246	WP_003403184.1	type II toxin-antitoxin system VapB family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-53)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14066.05 Da
• Isoelectric Point: 4.6558

>T25512 WP_014000320.1 NC_015848:c710367-709975 [Mycobacterium canettii CIPT 140010059]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAIVTQDNDYAAMPDVEVITI

Nucleotide

• Length: 393 bp

>T25512 NC_015848:c710367-709975 [Mycobacterium canettii CIPT 140010059]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCTGCCACAGCCGTGGC
GCACGGAATTGCGATCGTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9681.06 Da
• Isoelectric Point: 7.0175

>AT25512 WP_003403125.1 NC_015848:c710621-710364 [Mycobacterium canettii CIPT 140010059]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Nucleotide

• Length: 258 bp

>AT25512 NC_015848:c710621-710364 [Mycobacterium canettii CIPT 140010059]
ATGTCTGCTACGATTCCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA

Structures

Toxin

• AlphaFold DB: G0TQ85

Antitoxin

• AlphaFold DB: G0TQ86