Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 1982..2541 Replicon plasmid pMB9698_2
Accession NZ_CP103470
Organism Escherichia coli strain 4973

Toxin (Protein)


Gene name relE Uniprot ID U9YB49
Locus tag M5S50_RS25635 Protein ID WP_000421259.1
Coordinates 2266..2541 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A7Z1D1G5
Locus tag M5S50_RS25630 Protein ID WP_053264324.1
Coordinates 1982..2266 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S50_RS25625 (46) 46..1077 + 1032 WP_000907869.1 plasmid replication initiator RepA -
M5S50_RS25630 (1982) 1982..2266 + 285 WP_053264324.1 ribbon-helix-helix domain-containing protein Antitoxin
M5S50_RS25635 (2266) 2266..2541 + 276 WP_000421259.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M5S50_RS25640 (2647) 2647..2850 + 204 Protein_3 hypothetical protein -
M5S50_RS25645 (3120) 3120..3386 - 267 WP_021513970.1 hypothetical protein -
M5S50_RS25650 (3386) 3386..3880 - 495 WP_015058884.1 hypothetical protein -
M5S50_RS25655 (3936) 3936..4538 - 603 WP_000521606.1 ProQ/FINO family protein -
M5S50_RS25660 (4714) 4714..5766 - 1053 WP_000655519.1 hypothetical protein -
M5S50_RS25665 (5979) 5979..6569 - 591 WP_259423644.1 hypothetical protein -
M5S50_RS25670 (6569) 6569..6823 - 255 WP_000343103.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..85055 85055


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10736.45 Da        Isoelectric Point: 9.9324

>T255119 WP_000421259.1 NZ_CP103470:2266-2541 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Download         Length: 276 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10502.95 Da        Isoelectric Point: 5.6643

>AT255119 WP_053264324.1 NZ_CP103470:1982-2266 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
AESLSTDNPLPVPR

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9YB49

Antitoxin

Source ID Structure
AlphaFold DB A0A7Z1D1G5

References