Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4594747..4595244 Replicon chromosome
Accession NZ_CP103468
Organism Escherichia coli strain 4973

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag M5S50_RS22275 Protein ID WP_001173343.1
Coordinates 4594747..4594920 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag M5S50_RS22280 Protein ID WP_000398619.1
Coordinates 4594948..4595244 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S50_RS22265 (4591417) 4591417..4592874 + 1458 WP_022646457.1 dipeptide/tripeptide permease DtpC -
M5S50_RS22270 (4593111) 4593111..4594628 + 1518 WP_022646456.1 lysine--tRNA ligase -
M5S50_RS22275 (4594747) 4594747..4594920 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
M5S50_RS22280 (4594948) 4594948..4595244 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
M5S50_RS22285 (4595473) 4595473..4595745 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
M5S50_RS22290 (4595757) 4595757..4595986 - 230 Protein_4367 (4Fe-4S)-binding protein -
M5S50_RS22295 (4596167) 4596167..4597798 + 1632 WP_022646455.1 sensor histidine kinase -
M5S50_RS22300 (4597795) 4597795..4598514 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4544486..4595244 50758


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T255115 WP_001173343.1 NZ_CP103468:c4594920-4594747 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT255115 WP_000398619.1 NZ_CP103468:c4595244-4594948 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References