Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4344185..4344761 Replicon chromosome
Accession NZ_CP103468
Organism Escherichia coli strain 4973

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag M5S50_RS21045 Protein ID WP_001297643.1
Coordinates 4344474..4344761 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag M5S50_RS21040 Protein ID WP_000063156.1
Coordinates 4344185..4344487 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S50_RS21025 (4340781) 4340781..4342931 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
M5S50_RS21030 (4342981) 4342981..4343184 + 204 WP_000467859.1 YbdD/YjiX family protein -
M5S50_RS21035 (4343195) 4343195..4344151 + 957 WP_001297640.1 GTPase -
M5S50_RS21040 (4344185) 4344185..4344487 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
M5S50_RS21045 (4344474) 4344474..4344761 - 288 WP_001297643.1 BrnT family toxin Toxin
M5S50_RS21050 (4344960) 4344960..4345657 - 698 Protein_4124 IS1 family transposase -
M5S50_RS21055 (4345714) 4345714..4345809 + 96 Protein_4125 dienelactone hydrolase family protein -
M5S50_RS21060 (4345955) 4345955..4347187 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
M5S50_RS21065 (4347228) 4347228..4348508 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T255113 WP_001297643.1 NZ_CP103468:c4344761-4344474 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT255113 WP_000063156.1 NZ_CP103468:c4344487-4344185 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References