Detailed information of TA system TA025511

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 692803..693422
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPU5
• Locus tag: MCAN_RS03050
• Protein ID: WP_014000311.1
• Coordinates: 693015..693422 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPU4
• Locus tag: MCAN_RS03045
• Protein ID: WP_014000310.1
• Coordinates: 692803..693018 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS22665	688976..689122	+	147	WP_158309793.1	hypothetical protein	-
MCAN_RS03025	689143..690447	+	1305	WP_014000306.1	TIGR03086 family protein	-
MCAN_RS03030	690461..691246	+	786	WP_014000307.1	VOC family protein	-
MCAN_RS03035	691331..692089	+	759	WP_014000308.1	Mut7-C ubiquitin/RNAse domain-containing protein	-
MCAN_RS03040	692218..692709	-	492	WP_014000309.1	hypothetical protein	-
MCAN_RS03045	692803..693018	+	216	WP_014000310.1	type II toxin-antitoxin system antitoxin VapB26	Antitoxin
MCAN_RS03050	693015..693422	+	408	WP_014000311.1	type II toxin-antitoxin system toxin ribonuclease C26	Toxin
MCAN_RS03055	693482..694156	-	675	WP_014000312.1	LpqN/LpqT family lipoprotein	-
MCAN_RS03060	694310..696943	+	2634	WP_014000313.1	GH92 family glycosyl hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Ribbon-helix-helix protein, CopG (4-39)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14442.40 Da
• Isoelectric Point: 4.5746

>T25511 WP_014000311.1 NC_015848:693015-693422 [Mycobacterium canettii CIPT 140010059]
VIVDTSALLAYFDAAEPDHAAVSECIDSSADALVVSPYVVAELDYLVATRVGVDAELAVLRELAGGAWELANCGAAEIEQ
AARIGKKYQDQRIGIADAANVVLADRYRTRTILTLDRRHFSALRPIGGGRFTVIP

Nucleotide

• Length: 408 bp

>T25511 NC_015848:693015-693422 [Mycobacterium canettii CIPT 140010059]
GTGATCGTCGACACGAGTGCGCTGCTTGCCTATTTCGACGCCGCCGAGCCAGACCACGCCGCAGTGTCTGAGTGCATCGA
TAGCTCCGCAGACGCGCTCGTCGTATCCCCTTATGTTGTAGCGGAACTCGACTATCTCGTCGCCACCCGGGTAGGTGTCG
ATGCCGAGCTCGCCGTCCTGCGTGAACTCGCCGGCGGGGCTTGGGAGCTCGCCAACTGCGGTGCCGCCGAAATCGAGCAG
GCCGCCCGCATCGGCAAGAAATACCAGGATCAGCGGATCGGGATCGCGGATGCGGCCAACGTCGTGCTGGCCGACCGATA
CCGCACGCGCACGATCCTCACCCTGGACCGTCGGCACTTCTCGGCGCTGCGGCCGATCGGCGGTGGGCGCTTCACCGTCA
TTCCGTAA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7554.60 Da
• Isoelectric Point: 9.7768

>AT25511 WP_014000310.1 NC_015848:692803-693018 [Mycobacterium canettii CIPT 140010059]
VDKTTVYLPDELKAAVKHAARQRGVSEAEVIRESIRAAVGGAKPPPRGGLYAGSEPIARRVDELLAGFGER

Nucleotide

• Length: 216 bp

>AT25511 NC_015848:692803-693018 [Mycobacterium canettii CIPT 140010059]
GTGGACAAGACGACGGTCTACCTGCCGGATGAACTCAAGGCGGCCGTGAAGCACGCCGCTCGGCAGCGCGGAGTCTCCGA
AGCGGAGGTAATCCGGGAGTCCATCCGGGCGGCGGTCGGCGGCGCCAAGCCGCCGCCGCGCGGGGGTCTATATGCGGGTT
CGGAGCCCATCGCGCGGCGAGTCGACGAGCTGCTGGCTGGCTTCGGTGAGCGGTGA

Structures

Toxin

• AlphaFold DB: G0TPU5

Antitoxin

• AlphaFold DB: G0TPU4