Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3065784..3066568 Replicon chromosome
Accession NZ_CP103468
Organism Escherichia coli strain 4973

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag M5S50_RS14725 Protein ID WP_000613626.1
Coordinates 3066074..3066568 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A0A1AEM7
Locus tag M5S50_RS14720 Protein ID WP_024946541.1
Coordinates 3065784..3066077 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S50_RS14710 (3060945) 3060945..3061904 - 960 WP_022645527.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
M5S50_RS14715 (3062477) 3062477..3065650 + 3174 WP_022645526.1 ribonuclease E -
M5S50_RS14720 (3065784) 3065784..3066077 + 294 WP_024946541.1 DUF1778 domain-containing protein Antitoxin
M5S50_RS14725 (3066074) 3066074..3066568 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
M5S50_RS14730 (3066663) 3066663..3067616 - 954 WP_022645524.1 flagellar hook-associated protein FlgL -
M5S50_RS14735 (3067628) 3067628..3069271 - 1644 WP_022645523.1 flagellar hook-associated protein FlgK -
M5S50_RS14740 (3069337) 3069337..3070278 - 942 WP_001317765.1 flagellar assembly peptidoglycan hydrolase FlgJ -
M5S50_RS14745 (3070278) 3070278..3071375 - 1098 WP_021580666.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T255107 WP_000613626.1 NZ_CP103468:3066074-3066568 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11039.60 Da        Isoelectric Point: 10.5423

>AT255107 WP_024946541.1 NZ_CP103468:3065784-3066077 [Escherichia coli]
MAAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRSEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB A0A0A1AEM7

References