Detailed information of TA system TA025510

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 653700..654376
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: MCAN_RS02860
• Protein ID: WP_003898500.1
• Coordinates: 653700..654113 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: MCAN_RS02865
• Protein ID: WP_003402915.1
• Coordinates: 654110..654376 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS02830	649045..649347	-	303	WP_003402896.1	DUF3349 domain-containing protein	-
MCAN_RS02835	649407..649685	-	279	WP_003402901.1	hypothetical protein	-
MCAN_RS02840	649682..650935	-	1254	WP_014000281.1	inorganic phosphate transporter family protein	-
MCAN_RS02845	651055..651441	-	387	WP_003402907.1	VOC family protein	-
MCAN_RS02850	651504..652388	-	885	WP_014000282.1	SDR family oxidoreductase	-
MCAN_RS02855	652484..653386	-	903	WP_003915439.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
MCAN_RS02860	653700..654113	-	414	WP_003898500.1	PIN domain-containing protein	Toxin
MCAN_RS02865	654110..654376	-	267	WP_003402915.1	antitoxin VapB3	Antitoxin
MCAN_RS02870	654568..656283	-	1716	WP_014000283.1	fatty-acid--CoA ligase FadD8	-
MCAN_RS02875	656361..657965	+	1605	WP_014000284.1	amidohydrolase	-
MCAN_RS02880	657962..658942	+	981	WP_014000285.1	o-succinylbenzoate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T25510 WP_003898500.1 NC_015848:c654113-653700 [Mycobacterium canettii CIPT 140010059]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T25510 NC_015848:c654113-653700 [Mycobacterium canettii CIPT 140010059]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCCGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT25510 WP_003402915.1 NC_015848:c654376-654110 [Mycobacterium canettii CIPT 140010059]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT25510 NC_015848:c654376-654110 [Mycobacterium canettii CIPT 140010059]
TTGTTGAGCCGGCGCACGAAAACGATCGTTGTGTGTACATTGGTGTGTATGGCTCGGTTGAACGTGTATGTGCCCGACGA
ATTGGCGGAGCGCGCCAGGGCGCGGGGCTTGAACGTCTCGGCGCTGACTCAGGCCGCGATCAGTGCCGAGTTGGAGAACT
CCGCAACCGATGCGTGGCTTGAGGGGTTGGAACCCAGAAGCACCGGCGCTCGGCATGATGACGTGCTGGGTGCGATCGAT
GCCGCTCGCGATGAGTTCGAAGCGTGA

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0