255089

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3972147..3972723 Replicon chromosome
Accession NZ_CP103465
Organism Escherichia coli O25b:H4-ST131 strain 5392

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag M5T42_RS19295 Protein ID WP_001297643.1
Coordinates 3972436..3972723 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag M5T42_RS19290 Protein ID WP_000063156.1
Coordinates 3972147..3972449 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T42_RS19275 (3968743) 3968743..3970893 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
M5T42_RS19280 (3970943) 3970943..3971146 + 204 WP_000467859.1 YbdD/YjiX family protein -
M5T42_RS19285 (3971157) 3971157..3972113 + 957 WP_001297640.1 GTPase -
M5T42_RS19290 (3972147) 3972147..3972449 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
M5T42_RS19295 (3972436) 3972436..3972723 - 288 WP_001297643.1 BrnT family toxin Toxin
M5T42_RS19300 (3972922) 3972922..3973619 - 698 Protein_3779 IS1 family transposase -
M5T42_RS19305 (3973676) 3973676..3973771 + 96 Protein_3780 dienelactone hydrolase family protein -
M5T42_RS19310 (3973917) 3973917..3975149 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
M5T42_RS19315 (3975190) 3975190..3976470 + 1281 WP_001037382.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T255089 WP_001297643.1 NZ_CP103465:c3972723-3972436 [Escherichia coli O25b:H4-ST131]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT255089 WP_000063156.1 NZ_CP103465:c3972449-3972147 [Escherichia coli O25b:H4-ST131]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References