255088

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3862436..3862986 Replicon chromosome
Accession NZ_CP103465
Organism Escherichia coli O25b:H4-ST131 strain 5392

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag M5T42_RS18755 Protein ID WP_001160966.1
Coordinates 3862436..3862750 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag M5T42_RS18760 Protein ID WP_000125566.1
Coordinates 3862753..3862986 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T42_RS18730 (3858111) 3858111..3859397 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
M5T42_RS18735 (3859397) 3859397..3860386 + 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
M5T42_RS18740 (3860383) 3860383..3861204 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
M5T42_RS18745 (3861207) 3861207..3861584 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
M5T42_RS18750 (3861591) 3861591..3862439 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
M5T42_RS18755 (3862436) 3862436..3862750 - 315 WP_001160966.1 CcdB family protein Toxin
M5T42_RS18760 (3862753) 3862753..3862986 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
M5T42_RS18765 (3863072) 3863072..3863551 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
M5T42_RS18770 (3863743) 3863743..3865605 - 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
M5T42_RS18775 (3865598) 3865598..3866128 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
M5T42_RS18780 (3866236) 3866236..3867567 - 1332 WP_001183207.1 MFS transporter -
M5T42_RS18785 (3867626) 3867626..3867913 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T255088 WP_001160966.1 NZ_CP103465:c3862750-3862436 [Escherichia coli O25b:H4-ST131]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT255088 WP_000125566.1 NZ_CP103465:c3862986-3862753 [Escherichia coli O25b:H4-ST131]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References