Detailed information of TA system TA255087

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 3632901..3634590
• Replicon: chromosome
• Accession: NZ_CP103465
• Organism: Escherichia coli O25b:H4-ST131 strain 5392

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A061KTE0
• Locus tag: M5T42_RS17680
• Protein ID: WP_000365807.1
• Coordinates: 3632901..3633821 (-)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: M5T42_RS17685
• Protein ID: WP_001295795.1
• Coordinates: 3633808..3634590 (-)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M5T42_RS17640 (3628374)	3628374..3629084	+	711	WP_001295798.1	fimbrial chaperone EcpE	-
M5T42_RS17645 (3629391)	3629391..3629720	+	330	WP_001295797.1	YkgJ family cysteine cluster protein	-
M5T42_RS17650 (3629732)	3629732..3629898	-	167	Protein_3460	tail fiber assembly protein	-
M5T42_RS17655 (3629969)	3629969..3630583	-	615	WP_001019923.1	YagU family protein	-
M5T42_RS17660 (3630798)	3630798..3630983	-	186	WP_000146240.1	hypothetical protein	-
M5T42_RS17665 (3631032)	3631032..3631190	+	159	Protein_3463	integrase	-
M5T42_RS17670 (3631417)	3631417..3631752	+	336	WP_000996167.1	Arm DNA-binding domain-containing protein	-
M5T42_RS17675 (3631960)	3631960..3632736	+	777	WP_000171155.1	site-specific integrase	-
M5T42_RS17680 (3632901)	3632901..3633821	-	921	WP_000365807.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
M5T42_RS17685 (3633808)	3633808..3634590	-	783	WP_001295795.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
M5T42_RS17690 (3634874)	3634874..3637972	+	3099	WP_000673070.1	type I restriction endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	3615455..3641653	26198

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-223)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (3-90)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34570.89 Da
• Isoelectric Point: 7.3239

>T255087 WP_000365807.1 NZ_CP103465:c3633821-3632901 [Escherichia coli O25b:H4-ST131]
MDLRDSYARQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLGRPGLTREIFDGFLCYLAGHPRPIAELLASNWDTDRIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHFTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAMLKDKNPAKYVAAVNKLERVLE

Nucleotide

• Length: 921 bp

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29099.24 Da
• Isoelectric Point: 8.5975

>AT255087 WP_001295795.1 NZ_CP103465:c3634590-3633808 [Escherichia coli O25b:H4-ST131]
MPNYLNWLMQNTLPGQVLLQSWLTRNGVDRKLSYLYVQNGWLRRIAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLT
SLALQGHAHYTEPGKPQVWLALPAYVKLPGWFAAFEQSATFITLYTSGLTVDVSSFTTELKIGDRQLAGSAPELAAYEIA
CGVPSLISFEHADALFQGGNLLSPRKLQALLRASHSIKTNRVMLYLARRNGHPYFQYLDQSGIETGTGKRQIIPGGWLEP
DYQITVPRTFKPEGVEDGSA

Nucleotide

• Length: 783 bp

Structures

Toxin

• AlphaFold DB: A0A061KTE0