Detailed information of TA system TA025508

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 339469..340178
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TN77
• Locus tag: MCAN_RS01495
• Protein ID: WP_014000147.1
• Coordinates: 339469..339897 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MCAN_RS01500
• Protein ID: WP_041180000.1
• Coordinates: 339921..340178 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS01470	335336..336469	-	1134	WP_003401394.1	hypothetical protein	-
MCAN_RS01475	336466..337086	-	621	WP_003401397.1	TetR/AcrR family transcriptional regulator	-
MCAN_RS01480	337183..337764	+	582	WP_014000145.1	VOC family protein	-
MCAN_RS01485	337694..338419	-	726	WP_003902925.1	TetR/AcrR family transcriptional regulator	-
MCAN_RS01490	338509..339429	+	921	WP_014000146.1	DUF2236 domain-containing protein	-
MCAN_RS01495	339469..339897	-	429	WP_014000147.1	type II toxin-antitoxin system VapC family toxin	Toxin
MCAN_RS01500	339921..340178	-	258	WP_041180000.1	ribbon-helix-helix protein, CopG family	Antitoxin
MCAN_RS01505	340206..342395	-	2190	WP_014000148.1	PE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-141)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15878.28 Da
• Isoelectric Point: 7.2213

>T25508 WP_014000147.1 NC_015848:c339897-339469 [Mycobacterium canettii CIPT 140010059]
MFLLDANVLLAAHRGDHPNHRTVRPWFDRLLAADDPFTVPNLVWASFLRLTTNRRIFEIPSPRADAFAFVDAVTAQPHHL
PTSPGPRHLMLLRKLCDEADASGDLIPDAVLAAIAVEHHCAVVSLDRDFARFASVRHIRPPI

Nucleotide

• Length: 429 bp

>T25508 NC_015848:c339897-339469 [Mycobacterium canettii CIPT 140010059]
ATGTTCCTTCTCGACGCCAACGTGCTGCTGGCGGCACACCGCGGTGACCATCCGAATCACCGAACCGTCCGCCCCTGGTT
CGATCGACTGCTTGCGGCCGACGACCCCTTCACAGTGCCGAACCTAGTGTGGGCGTCGTTCCTCCGGCTAACAACGAATC
GACGCATCTTCGAGATTCCGTCACCACGAGCGGATGCATTCGCATTCGTCGACGCCGTCACCGCCCAGCCCCATCACCTT
CCGACAAGCCCCGGCCCCAGACACCTCATGCTGCTGCGAAAACTCTGCGACGAGGCCGACGCATCGGGCGACTTGATACC
TGACGCGGTACTCGCGGCCATCGCCGTGGAGCATCACTGCGCCGTGGTGAGCCTGGATAGGGATTTCGCCCGGTTTGCCT
CTGTGCGCCATATTCGCCCGCCAATCTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9065.24 Da
• Isoelectric Point: 8.9952

>AT25508 WP_041180000.1 NC_015848:c340178-339921 [Mycobacterium canettii CIPT 140010059]
MRTTVSISDELLAAAKRRARERGQSLGAVIEDALRRELAAAHTGGARPTVPVFDAGTGPRPGIDLTSNRVLSEVLDEGLE
LNSRK

Nucleotide

• Length: 258 bp

>AT25508 NC_015848:c340178-339921 [Mycobacterium canettii CIPT 140010059]
ATGCGCACCACCGTGTCAATCTCGGATGAACTACTGGCTGCCGCCAAACGCCGAGCCCGCGAGCGTGGTCAATCGCTGGG
CGCTGTGATCGAAGACGCCCTTCGGCGGGAGCTCGCCGCCGCCCACACCGGCGGCGCCCGGCCAACCGTCCCCGTTTTTG
ACGCCGGCACCGGTCCGCGGCCAGGTATCGACCTGACCTCGAATAGGGTGTTGTCCGAAGTGCTTGACGAGGGCCTGGAA
CTGAACTCCCGGAAGTAA

Structures

Toxin

• AlphaFold DB: G0TN77