255071

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 290780..291580 Replicon chromosome
Accession NZ_CP103465
Organism Escherichia coli O25b:H4-ST131 strain 5392

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag M5T42_RS01355 Protein ID WP_000342452.1
Coordinates 291053..291580 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag M5T42_RS01350 Protein ID WP_001277107.1
Coordinates 290780..291046 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T42_RS01330 (286439) 286439..287107 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
M5T42_RS01335 (287100) 287100..288158 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
M5T42_RS01340 (288403) 288403..289257 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
M5T42_RS01345 (289528) 289528..290631 + 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
M5T42_RS01350 (290780) 290780..291046 + 267 WP_001277107.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
M5T42_RS01355 (291053) 291053..291580 + 528 WP_000342452.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
M5T42_RS01360 (291577) 291577..291960 - 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
M5T42_RS01365 (292383) 292383..293492 + 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
M5T42_RS01370 (293540) 293540..294466 + 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
M5T42_RS01375 (294463) 294463..295740 + 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
M5T42_RS01380 (295737) 295737..296504 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T255071 WP_000342452.1 NZ_CP103465:291053-291580 [Escherichia coli O25b:H4-ST131]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT255071 WP_001277107.1 NZ_CP103465:290780-291046 [Escherichia coli O25b:H4-ST131]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References