Detailed information of TA system TA025507

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 73650..74283
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TM77
• Locus tag: MCAN_RS00370
• Protein ID: WP_014000017.1
• Coordinates: 73882..74283 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MCAN_RS00365
• Protein ID: WP_015287750.1
• Coordinates: 73650..73889 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS20850	68839..69375	-	537	WP_014000015.1	MerR family DNA-binding protein	-
MCAN_RS00355	69640..70232	+	593	Protein_67	hypothetical protein	-
MCAN_RS20860	70442..70603	+	162	WP_003899796.1	hypothetical protein	-
MCAN_RS00360	70644..73583	+	2940	WP_014000016.1	UPF0182 family protein	-
MCAN_RS00365	73650..73889	+	240	WP_015287750.1	hypothetical protein	Antitoxin
MCAN_RS00370	73882..74283	+	402	WP_014000017.1	type II toxin-antitoxin system ribonuclease VapC1	Toxin
MCAN_RS00375	74335..76572	-	2238	WP_014000018.1	NADP-dependent isocitrate dehydrogenase	-
MCAN_RS00380	76690..77259	-	570	WP_003400591.1	TetR/AcrR family transcriptional regulator; helix-turn-helix transcriptional regulator	-
MCAN_RS00385	77362..78273	+	912	WP_003899798.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (36-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14311.39 Da
• Isoelectric Point: 4.8887

>T25507 WP_014000017.1 NC_015848:73882-74283 [Mycobacterium canettii CIPT 140010059]
VDECVVDAAAAVDALAGKGASAIVLRGLLKESISNAPHLLDAEVGHALRRAVLSDEISEEQARAALDALPYLIDNRYPHS
PRLIEYTWQLRHNVTFYDALYVALATALDVPLLTGDSRLAAAPGLPCEIKLVR

Nucleotide

• Length: 402 bp

>T25507 NC_015848:73882-74283 [Mycobacterium canettii CIPT 140010059]
GTGGATGAATGTGTAGTCGACGCGGCGGCCGCGGTTGACGCTCTCGCCGGCAAGGGCGCCAGCGCGATCGTTCTGCGCGG
TTTGCTCAAGGAGTCGATTTCTAACGCGCCGCATTTGCTGGACGCAGAGGTCGGACATGCACTCCGCCGCGCCGTGCTCA
GCGACGAAATCTCCGAAGAGCAGGCTCGCGCCGCGTTGGATGCCTTGCCTTATCTCATCGACAATCGTTACCCGCACAGC
CCACGACTGATCGAATACACATGGCAGCTAAGGCACAATGTCACGTTCTACGACGCCCTTTACGTAGCACTGGCCACCGC
ACTGGATGTCCCGCTGCTCACGGGCGACTCGCGGCTTGCGGCCGCGCCGGGCCTTCCGTGCGAAATCAAACTCGTTCGGT
GA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8689.79 Da
• Isoelectric Point: 8.5960

>AT25507 WP_015287750.1 NC_015848:73650-73889 [Mycobacterium canettii CIPT 140010059]
MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEGARGRDKAEAIEILEQALASTASPGISRETIEASRRELRGG

Nucleotide

• Length: 240 bp

>AT25507 NC_015848:73650-73889 [Mycobacterium canettii CIPT 140010059]
ATGGCTACCATTCAAGTTCGGGATTTGCCCGAAGATGTCGCCGAAACCTATCGACGGCGCGCCACCGCAGCGGGGCAGTC
GCTGCAGACGTATATGCGCACCAAGCTCATCGAAGGGGCGCGGGGCCGAGACAAGGCCGAGGCAATCGAGATCCTGGAAC
AGGCGCTCGCCAGCACTGCCAGCCCAGGCATCAGCCGGGAGACCATCGAGGCATCCCGGCGGGAGCTCAGGGGTGGATGA

Structures

Toxin

• AlphaFold DB: G0TM77