255069

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3611007..3611643 Replicon chromosome
Accession NZ_CP103456
Organism Bacillus subtilis strain PN176 (HK176)

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag NX050_RS19435 Protein ID WP_003156187.1
Coordinates 3611007..3611357 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag NX050_RS19440 Protein ID WP_003225183.1
Coordinates 3611362..3611643 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NX050_RS19395 (NX050_19395) 3606043..3606642 - 600 WP_003246608.1 phosphoserine phosphatase RsbX -
NX050_RS19400 (NX050_19400) 3606642..3607430 - 789 WP_003246715.1 RNA polymerase sigma factor SigB -
NX050_RS19405 (NX050_19405) 3607396..3607878 - 483 WP_003234299.1 anti-sigma B factor RsbW -
NX050_RS19410 (NX050_19410) 3607875..3608204 - 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
NX050_RS19415 (NX050_19415) 3608273..3609280 - 1008 WP_014478900.1 phosphoserine phosphatase RsbU -
NX050_RS19420 (NX050_19420) 3609292..3609693 - 402 WP_014478899.1 serine/threonine-protein kinase RsbT -
NX050_RS19425 (NX050_19425) 3609697..3610062 - 366 WP_003225190.1 RsbT antagonist protein RsbS -
NX050_RS19430 (NX050_19430) 3610067..3610891 - 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
NX050_RS19435 (NX050_19435) 3611007..3611357 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NX050_RS19440 (NX050_19440) 3611362..3611643 - 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
NX050_RS19445 (NX050_19445) 3611759..3612928 - 1170 WP_014478898.1 alanine racemase -
NX050_RS19450 (NX050_19450) 3613043..3614059 - 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
NX050_RS19455 (NX050_19455) 3614225..3614590 - 366 WP_014478897.1 holo-ACP synthase -
NX050_RS19460 (NX050_19460) 3614685..3615284 + 600 WP_014478896.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T255069 WP_003156187.1 NZ_CP103456:c3611357-3611007 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT255069 WP_003225183.1 NZ_CP103456:c3611643-3611362 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB G4NU32

References