Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 2759620..2760536 Replicon chromosome
Accession NZ_CP103456
Organism Bacillus subtilis strain PN176 (HK176)

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag NX050_RS14815 Protein ID WP_003244695.1
Coordinates 2759620..2760366 (+) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag NX050_RS14820 Protein ID WP_003232646.1
Coordinates 2760366..2760536 (+) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NX050_RS14790 (NX050_14790) 2754744..2754839 - 96 Protein_2919 hypothetical protein -
NX050_RS14795 (NX050_14795) 2754948..2755898 - 951 WP_014479571.1 ring-cleaving dioxygenase -
NX050_RS14800 (NX050_14800) 2756287..2757603 + 1317 WP_014479570.1 serine/threonine exchanger -
NX050_RS14805 (NX050_14805) 2757879..2758496 + 618 WP_003218470.1 DUF47 domain-containing protein -
NX050_RS14810 (NX050_14810) 2758509..2759510 + 1002 WP_014479569.1 inorganic phosphate transporter -
NX050_RS14815 (NX050_14815) 2759620..2760366 + 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
NX050_RS14820 (NX050_14820) 2760366..2760536 + 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
NX050_RS14825 (NX050_14825) 2760622..2760759 + 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
NX050_RS14830 (NX050_14830) 2760797..2761690 - 894 WP_014479567.1 N-acetylmuramoyl-L-alanine amidase -
NX050_RS14835 (NX050_14835) 2761703..2761966 - 264 WP_014479566.1 phage holin -
NX050_RS14840 (NX050_14840) 2761979..2762248 - 270 WP_014479565.1 hemolysin XhlA family protein -
NX050_RS14845 (NX050_14845) 2762301..2763140 - 840 WP_014479564.1 phage-like element PBSX protein XepA -
NX050_RS14850 (NX050_14850) 2763187..2763351 - 165 WP_014479563.1 XkdX family protein -
NX050_RS14855 (NX050_14855) 2763348..2763677 - 330 WP_014479562.1 XkdW family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T255068 WP_003244695.1 NZ_CP103456:2759620-2760366 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT255068 WP_003232646.1 NZ_CP103456:2760366-2760536 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References