Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5534660..5535285 Replicon chromosome
Accession NZ_CP103420
Organism Klebsiella variicola strain KV1

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag NYO12_RS27360 Protein ID WP_008807903.1
Coordinates 5534660..5535043 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NYO12_RS27365 Protein ID WP_004150355.1
Coordinates 5535043..5535285 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NYO12_RS27345 (5532026) 5532026..5532928 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NYO12_RS27350 (5532925) 5532925..5533560 + 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
NYO12_RS27355 (5533557) 5533557..5534486 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NYO12_RS27360 (5534660) 5534660..5535043 - 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
NYO12_RS27365 (5535043) 5535043..5535285 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NYO12_RS27370 (5535490) 5535490..5536407 + 918 WP_259072597.1 alpha/beta hydrolase -
NYO12_RS27375 (5536422) 5536422..5537363 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
NYO12_RS27380 (5537408) 5537408..5537845 - 438 WP_012543288.1 D-aminoacyl-tRNA deacylase -
NYO12_RS27385 (5537842) 5537842..5538702 - 861 WP_008807907.1 virulence factor BrkB family protein -
NYO12_RS27390 (5538696) 5538696..5539295 - 600 WP_008807908.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T255040 WP_008807903.1 NZ_CP103420:c5535043-5534660 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT255040 WP_004150355.1 NZ_CP103420:c5535285-5535043 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References