Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1791891..1792481 Replicon chromosome
Accession NZ_CP103419
Organism Klebsiella variicola strain KV4

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag NYO13_RS08585 Protein ID WP_008804165.1
Coordinates 1792149..1792481 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1F2LZQ3
Locus tag NYO13_RS08580 Protein ID WP_012541132.1
Coordinates 1791891..1792148 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NYO13_RS08560 (NYO13_08560) 1787502..1788077 + 576 WP_048330527.1 hypothetical protein -
NYO13_RS08565 (NYO13_08565) 1788254..1789090 + 837 WP_044612973.1 alpha/beta hydrolase -
NYO13_RS08570 (NYO13_08570) 1789296..1790267 + 972 WP_016161433.1 sensor domain-containing diguanylate cyclase -
NYO13_RS08575 (NYO13_08575) 1790264..1791364 - 1101 WP_048330526.1 AarF/UbiB family protein -
NYO13_RS08580 (NYO13_08580) 1791891..1792148 + 258 WP_012541132.1 antitoxin Antitoxin
NYO13_RS08585 (NYO13_08585) 1792149..1792481 + 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
NYO13_RS08595 (NYO13_08595) 1792804..1794240 + 1437 WP_259145131.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
NYO13_RS08605 (NYO13_08605) 1794566..1794715 - 150 Protein_1683 antitoxin -
NYO13_RS08610 (NYO13_08610) 1794919..1795221 - 303 WP_032700907.1 XRE family transcriptional regulator -
NYO13_RS08615 (NYO13_08615) 1795226..1795579 - 354 WP_008804168.1 type II toxin-antitoxin system RelE/ParE family toxin -
NYO13_RS08620 (NYO13_08620) 1795783..1797264 + 1482 WP_008804169.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T255017 WP_008804165.1 NZ_CP103419:1792149-1792481 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9420.91 Da        Isoelectric Point: 4.4915

>AT255017 WP_012541132.1 NZ_CP103419:1791891-1792148 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZQ3

References