Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 276881..277512 Replicon chromosome
Accession NZ_CP103419
Organism Klebsiella variicola strain KV4

Toxin (Protein)


Gene name hicA Uniprot ID A0A2V3KJT3
Locus tag NYO13_RS01250 Protein ID WP_044650325.1
Coordinates 276881..277156 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID R4YIG2
Locus tag NYO13_RS01255 Protein ID WP_004181489.1
Coordinates 277153..277512 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NYO13_RS01230 (NYO13_01230) 273162..273860 - 699 WP_048330888.1 dimethylmenaquinone methyltransferase -
NYO13_RS01235 (NYO13_01235) 273871..274824 - 954 WP_004204934.1 hydroxyacid dehydrogenase -
NYO13_RS01240 (NYO13_01240) 275161..275436 + 276 WP_044650324.1 LysR family transcriptional regulator -
NYO13_RS01245 (NYO13_01245) 275471..276484 - 1014 WP_012540371.1 magnesium transporter -
NYO13_RS01250 (NYO13_01250) 276881..277156 + 276 WP_044650325.1 type II toxin-antitoxin system HicA family toxin Toxin
NYO13_RS01255 (NYO13_01255) 277153..277512 + 360 WP_004181489.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
NYO13_RS01260 (NYO13_01260) 277540..277941 - 402 WP_048330886.1 nickel-responsive transcriptional regulator NikR -
NYO13_RS01265 (NYO13_01265) 277929..278720 - 792 WP_012540373.1 nickel import ATP-binding protein NikE -
NYO13_RS01270 (NYO13_01270) 278717..279481 - 765 WP_008806946.1 nickel import ATP-binding protein NikD -
NYO13_RS01275 (NYO13_01275) 279481..280314 - 834 WP_016161925.1 nickel ABC transporter permease subunit NikC -
NYO13_RS01280 (NYO13_01280) 280311..281255 - 945 WP_008806948.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10279.91 Da        Isoelectric Point: 10.3165

>T255013 WP_044650325.1 NZ_CP103419:276881-277156 [Klebsiella variicola]
MEQQVLSLRNKQRHTLEQLFKIPVPQGIKWADIESLIKALGGEIKEGRGSRCKFLLNHSIASFHRPHPSPDTDKGAVESV
RDWLITIGVKP

Download         Length: 276 bp

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13313.02 Da        Isoelectric Point: 4.4605

>AT255013 WP_004181489.1 NZ_CP103419:277153-277512 [Klebsiella variicola]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQQEGEISLQEYLADCHEAGIEPYAHPEKMKT
FTLRYPESFGERLSSAAAEEQVSVNTWILETLNERLKQA

Download         Length: 360 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V3KJT3

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2M5Q1

References