255012

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 1279572..1280131 Replicon chromosome
Accession NZ_CP103417
Organism Brucella melitensis strain PB150210

Toxin (Protein)


Gene name brnT Uniprot ID A0A7U8PD14
Locus tag NU954_RS06370 Protein ID WP_002966807.1
Coordinates 1279886..1280131 (-) Length 82 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID C0RIV1
Locus tag NU954_RS06365 Protein ID WP_004683740.1
Coordinates 1279572..1279886 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NU954_RS06315 (NU954_06315) 1275277..1275480 - 204 WP_004683737.1 hypothetical protein -
NU954_RS06320 (NU954_06320) 1275483..1275698 - 216 WP_005968947.1 hypothetical protein -
NU954_RS06325 (NU954_06325) 1275695..1275898 - 204 WP_002964088.1 hypothetical protein -
NU954_RS06330 (NU954_06330) 1275948..1276652 + 705 WP_005968944.1 hypothetical protein -
NU954_RS06335 (NU954_06335) 1276649..1276879 + 231 WP_002964091.1 hypothetical protein -
NU954_RS06340 (NU954_06340) 1276876..1277151 + 276 WP_002964092.1 hypothetical protein -
NU954_RS06345 (NU954_06345) 1277243..1277650 + 408 WP_002971458.1 hypothetical protein -
NU954_RS06350 (NU954_06350) 1277996..1278706 + 711 WP_004683739.1 porin family protein -
NU954_RS06355 (NU954_06355) 1279054..1279224 - 171 WP_002964095.1 DUF1508 domain-containing protein -
NU954_RS06360 (NU954_06360) 1279223..1279456 + 234 WP_002969999.1 hypothetical protein -
NU954_RS06365 (NU954_06365) 1279572..1279886 - 315 WP_004683740.1 BrnA antitoxin family protein Antitoxin
NU954_RS06370 (NU954_06370) 1279886..1280131 - 246 WP_002966807.1 BrnT family toxin Toxin
NU954_RS06375 (NU954_06375) 1280264..1280902 + 639 Protein_1249 tRNA guanosine transglycosylase -
NU954_RS06380 (NU954_06380) 1280972..1281730 + 759 Protein_1250 IS5 family transposase -
NU954_RS06385 (NU954_06385) 1281731..1282504 + 774 Protein_1251 hypothetical protein -
NU954_RS06390 (NU954_06390) 1282835..1284286 + 1452 WP_006135932.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - wboA / wbdA 1273505..1287602 14097
- flank IS/Tn - - 1280972..1281340 368


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-78)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-102)


Sequences

Toxin        


Download         Length: 82 a.a.        Molecular weight: 9162.82 Da        Isoelectric Point: 10.3158

>T255012 WP_002966807.1 NZ_CP103417:c1280131-1279886 [Brucella melitensis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Download         Length: 246 bp

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11653.48 Da        Isoelectric Point: 10.5824

>AT255012 WP_004683740.1 NZ_CP103417:c1279886-1279572 [Brucella melitensis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDKQLAKAKPFKESFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Download         Length: 315 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U8PD14

Antitoxin

Source ID Structure
AlphaFold DB C0RIV1

References