Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 485416..486053 Replicon chromosome
Accession NZ_CP103412
Organism Bacillus velezensis strain HF-14109

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag NX856_RS02495 Protein ID WP_003156187.1
Coordinates 485703..486053 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag NX856_RS02490 Protein ID WP_003156188.1
Coordinates 485416..485697 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NX856_RS02470 (481781) 481781..482380 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
NX856_RS02475 (482473) 482473..482838 + 366 WP_007410229.1 holo-ACP synthase -
NX856_RS02480 (483003) 483003..484010 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
NX856_RS02485 (484127) 484127..485296 + 1170 WP_025284272.1 alanine racemase -
NX856_RS02490 (485416) 485416..485697 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
NX856_RS02495 (485703) 485703..486053 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NX856_RS02500 (486171) 486171..486992 + 822 WP_003156182.1 STAS domain-containing protein -
NX856_RS02505 (486997) 486997..487362 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
NX856_RS02510 (487365) 487365..487766 + 402 WP_003156178.1 anti-sigma regulatory factor -
NX856_RS02515 (487778) 487778..488785 + 1008 WP_052827076.1 PP2C family protein-serine/threonine phosphatase -
NX856_RS02520 (488849) 488849..489178 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
NX856_RS02525 (489175) 489175..489657 + 483 WP_003156173.1 anti-sigma B factor RsbW -
NX856_RS02530 (489623) 489623..490411 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
NX856_RS02535 (490411) 490411..491013 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T255007 WP_003156187.1 NZ_CP103412:485703-486053 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT255007 WP_003156188.1 NZ_CP103412:485416-485697 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References