Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09907-MazE
Location 963..1564 Replicon plasmid pPUL102_5
Accession NZ_CP103396
Organism Levilactobacillus brevis strain PL102

Toxin (Protein)


Gene name pemK Uniprot ID Q684P1
Locus tag NX820_RS12640 Protein ID WP_001748110.1
Coordinates 1220..1564 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A806J926
Locus tag NX820_RS12635 Protein ID WP_001748109.1
Coordinates 963..1226 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NX820_RS12630 (NX820_12630) 290..877 - 588 WP_050340451.1 site-specific integrase -
NX820_RS12635 (NX820_12635) 963..1226 + 264 WP_001748109.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
NX820_RS12640 (NX820_12640) 1220..1564 + 345 WP_001748110.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
NX820_RS12645 (NX820_12645) 1683..3014 - 1332 WP_050340448.1 NAD(P)/FAD-dependent oxidoreductase -
NX820_RS12650 (NX820_12650) 3032..3163 - 132 WP_015474697.1 hypothetical protein -
NX820_RS12655 (NX820_12655) 3176..3823 - 648 WP_006844821.1 DsbA family oxidoreductase -
NX820_RS12660 (NX820_12660) 3842..4786 - 945 WP_006844822.1 thioredoxin-disulfide reductase -
NX820_RS12665 (NX820_12665) 4877..5005 - 129 Protein_7 aspartate racemase -
NX820_RS12670 (NX820_12670) 5005..5328 - 324 WP_006844823.1 thioredoxin family protein -
NX820_RS12675 (NX820_12675) 5349..5663 - 315 WP_015474701.1 thioredoxin -
NX820_RS12680 (NX820_12680) 5677..5958 - 282 WP_008855846.1 thioredoxin family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..11390 11390


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin

Antitoxin PemI-like

(8-85)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13041.89 Da        Isoelectric Point: 6.4782

>T254989 WP_001748110.1 NZ_CP103396:1220-1564 [Levilactobacillus brevis]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Download         Length: 345 bp

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10141.47 Da        Isoelectric Point: 5.7117

>AT254989 WP_001748109.1 NZ_CP103396:963-1226 [Levilactobacillus brevis]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW

Download         Length: 264 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R2K1X3

Antitoxin

Source ID Structure
AlphaFold DB A0A806J926

References