Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1376514..1377430 Replicon chromosome
Accession NZ_CP103352
Organism Bacillus subtilis strain SRCM117508

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag NX823_RS07125 Protein ID WP_003244695.1
Coordinates 1376684..1377430 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag NX823_RS07120 Protein ID WP_003232646.1
Coordinates 1376514..1376684 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NX823_RS07085 (NX823_07085) 1373373..1373702 + 330 WP_046160312.1 XkdW family protein -
NX823_RS07090 (NX823_07090) 1373699..1373863 + 165 WP_014479563.1 XkdX family protein -
NX823_RS07095 (NX823_07095) 1373910..1374749 + 840 WP_014479564.1 phage-like element PBSX protein XepA -
NX823_RS07100 (NX823_07100) 1374802..1375071 + 270 WP_014479565.1 hemolysin XhlA family protein -
NX823_RS07105 (NX823_07105) 1375084..1375347 + 264 WP_014479566.1 phage holin -
NX823_RS07110 (NX823_07110) 1375360..1376253 + 894 WP_258996659.1 N-acetylmuramoyl-L-alanine amidase -
NX823_RS07115 (NX823_07115) 1376291..1376428 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
NX823_RS07120 (NX823_07120) 1376514..1376684 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
NX823_RS07125 (NX823_07125) 1376684..1377430 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
NX823_RS07130 (NX823_07130) 1377540..1378541 - 1002 WP_014479569.1 inorganic phosphate transporter -
NX823_RS07135 (NX823_07135) 1378554..1379171 - 618 WP_003218470.1 DUF47 domain-containing protein -
NX823_RS07140 (NX823_07140) 1379447..1380763 - 1317 WP_014479570.1 serine/threonine exchanger -
NX823_RS07145 (NX823_07145) 1381152..1382102 + 951 WP_014479571.1 ring-cleaving dioxygenase -
NX823_RS07150 (NX823_07150) 1382211..1382306 + 96 Protein_1345 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1337048..1385794 48746


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T254988 WP_003244695.1 NZ_CP103352:c1377430-1376684 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT254988 WP_003232646.1 NZ_CP103352:c1376684-1376514 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References