Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 530861..531497 Replicon chromosome
Accession NZ_CP103352
Organism Bacillus subtilis strain SRCM117508

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag NX823_RS02645 Protein ID WP_003156187.1
Coordinates 531147..531497 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag NX823_RS02640 Protein ID WP_003225183.1
Coordinates 530861..531142 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NX823_RS02620 (NX823_02620) 527220..527819 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
NX823_RS02625 (NX823_02625) 527914..528279 + 366 WP_014478897.1 holo-ACP synthase -
NX823_RS02630 (NX823_02630) 528445..529461 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
NX823_RS02635 (NX823_02635) 529576..530745 + 1170 WP_015252766.1 alanine racemase -
NX823_RS02640 (NX823_02640) 530861..531142 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
NX823_RS02645 (NX823_02645) 531147..531497 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NX823_RS02650 (NX823_02650) 531602..532426 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
NX823_RS02655 (NX823_02655) 532431..532796 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
NX823_RS02660 (NX823_02660) 532800..533201 + 402 WP_017697001.1 serine/threonine-protein kinase RsbT -
NX823_RS02665 (NX823_02665) 533213..534220 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
NX823_RS02670 (NX823_02670) 534282..534611 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
NX823_RS02675 (NX823_02675) 534608..535090 + 483 WP_003234299.1 anti-sigma B factor RsbW -
NX823_RS02680 (NX823_02680) 535056..535844 + 789 Protein_486 RNA polymerase sigma factor SigB -
NX823_RS02685 (NX823_02685) 535844..536443 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T254987 WP_003156187.1 NZ_CP103352:531147-531497 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT254987 WP_003225183.1 NZ_CP103352:530861-531142 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References