Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1373073..1373989 Replicon chromosome
Accession NZ_CP103351
Organism Bacillus subtilis strain SRCM115947

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag NX819_RS07300 Protein ID WP_003244695.1
Coordinates 1373243..1373989 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag NX819_RS07295 Protein ID WP_003232646.1
Coordinates 1373073..1373243 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NX819_RS07260 (NX819_07260) 1369936..1370265 + 330 WP_003232660.1 XkdW family protein -
NX819_RS07265 (NX819_07265) 1370262..1370426 + 165 WP_003232658.1 XkdX family protein -
NX819_RS07270 (NX819_07270) 1370470..1371309 + 840 WP_041850926.1 phage-like element PBSX protein XepA -
NX819_RS07275 (NX819_07275) 1371362..1371631 + 270 WP_003232655.1 hemolysin XhlA family protein -
NX819_RS07280 (NX819_07280) 1371644..1371907 + 264 WP_003232653.1 phage holin -
NX819_RS07285 (NX819_07285) 1371920..1372813 + 894 WP_003245230.1 N-acetylmuramoyl-L-alanine amidase -
NX819_RS07290 (NX819_07290) 1372850..1372987 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
NX819_RS07295 (NX819_07295) 1373073..1373243 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
NX819_RS07300 (NX819_07300) 1373243..1373989 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
NX819_RS07305 (NX819_07305) 1374099..1375100 - 1002 WP_003232642.1 inorganic phosphate transporter -
NX819_RS07310 (NX819_07310) 1375113..1375730 - 618 WP_003218470.1 DUF47 domain-containing protein -
NX819_RS07315 (NX819_07315) 1376006..1377322 - 1317 WP_003244819.1 serine/threonine exchanger -
NX819_RS07320 (NX819_07320) 1377711..1378661 + 951 WP_003245086.1 ring-cleaving dioxygenase -
NX819_RS07325 (NX819_07325) 1378762..1378908 + 147 WP_003244977.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T254984 WP_003244695.1 NZ_CP103351:c1373989-1373243 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT254984 WP_003232646.1 NZ_CP103351:c1373243-1373073 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References