Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 571294..571930 Replicon chromosome
Accession NZ_CP103351
Organism Bacillus subtilis strain SRCM115947

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag NX819_RS03020 Protein ID WP_003156187.1
Coordinates 571580..571930 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag NX819_RS03015 Protein ID WP_003225183.1
Coordinates 571294..571575 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NX819_RS02995 (NX819_02995) 567653..568252 - 600 WP_032722928.1 rhomboid family intramembrane serine protease -
NX819_RS03000 (NX819_03000) 568347..568712 + 366 WP_015252768.1 holo-ACP synthase -
NX819_RS03005 (NX819_03005) 568878..569894 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
NX819_RS03010 (NX819_03010) 570009..571178 + 1170 WP_015252766.1 alanine racemase -
NX819_RS03015 (NX819_03015) 571294..571575 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
NX819_RS03020 (NX819_03020) 571580..571930 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NX819_RS03025 (NX819_03025) 572045..572869 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
NX819_RS03030 (NX819_03030) 572874..573239 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
NX819_RS03035 (NX819_03035) 573243..573644 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
NX819_RS03040 (NX819_03040) 573656..574663 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
NX819_RS03045 (NX819_03045) 574725..575054 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
NX819_RS03050 (NX819_03050) 575051..575533 + 483 WP_003234299.1 anti-sigma B factor RsbW -
NX819_RS03055 (NX819_03055) 575499..576287 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
NX819_RS03060 (NX819_03060) 576287..576886 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T254983 WP_003156187.1 NZ_CP103351:571580-571930 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT254983 WP_003225183.1 NZ_CP103351:571294-571575 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References