Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 86541..88164 Replicon plasmid p1
Accession NZ_CP103330
Organism Escherichia coli strain ZY22049

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag NX000_RS23875 Protein ID WP_000088046.1
Coordinates 86839..88164 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag NX000_RS23870 Protein ID WP_024136327.1
Coordinates 86541..86834 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NX000_RS23840 (NX000_23775) 82414..83655 + 1242 WP_001053338.1 VWA domain-containing protein -
NX000_RS23845 (NX000_23780) 83746..83877 + 132 WP_000374059.1 hypothetical protein -
NX000_RS23850 (NX000_23785) 84218..84499 - 282 WP_000077926.1 hypothetical protein -
NX000_RS23855 (NX000_23790) 84549..84740 - 192 WP_000398480.1 hypothetical protein -
NX000_RS23860 (NX000_23795) 84832..85173 - 342 WP_001151575.1 hypothetical protein -
NX000_RS23865 (NX000_23800) 85546..85938 + 393 WP_000341066.1 hypothetical protein -
NX000_RS23870 (NX000_23805) 86541..86834 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
NX000_RS23875 (NX000_23810) 86839..88164 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
NX000_RS23880 (NX000_23815) 88225..88431 + 207 WP_000134171.1 hypothetical protein -
NX000_RS23885 (NX000_23820) 88533..88943 + 411 WP_000985911.1 hypothetical protein -
NX000_RS23890 (NX000_23825) 88956..89771 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
NX000_RS23895 (NX000_23830) 90025..90450 + 426 WP_001043843.1 hypothetical protein -
NX000_RS23900 (NX000_23835) 90999..91307 + 309 WP_001224687.1 hypothetical protein -
NX000_RS23905 (NX000_23840) 91323..92180 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
NX000_RS23910 (NX000_23845) 92242..92445 + 204 WP_001194555.1 hypothetical protein -
NX000_RS23915 (NX000_23850) 92787..93143 - 357 WP_065311124.1 H-NS family nucleoid-associated regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / floR / aph(6)-Id / tet(A) / sul3 / lnu(F) / ant(3'')-Ia / aac(3)-IId / blaCTX-M-55 / qnrS1 / mph(A) / ARR-3 / dfrA14 - 1..261664 261664


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T254948 WP_000088046.1 NZ_CP103330:86839-88164 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT254948 WP_024136327.1 NZ_CP103330:86541-86834 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References