Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 319509..320309 Replicon chromosome
Accession NZ_CP103329
Organism Escherichia coli strain ZY22049

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag NX000_RS01480 Protein ID WP_000342449.1
Coordinates 319782..320309 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag NX000_RS01475 Protein ID WP_001277108.1
Coordinates 319509..319775 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NX000_RS01455 (315166) 315166..315834 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
NX000_RS01460 (315827) 315827..316885 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
NX000_RS01465 (317130) 317130..317984 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
NX000_RS01470 (318256) 318256..319359 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
NX000_RS01475 (319509) 319509..319775 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
NX000_RS01480 (319782) 319782..320309 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
NX000_RS01485 (320306) 320306..320689 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
NX000_RS01490 (321113) 321113..322222 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
NX000_RS01495 (322270) 322270..323196 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
NX000_RS01500 (323193) 323193..324470 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
NX000_RS01505 (324467) 324467..325234 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T254932 WP_000342449.1 NZ_CP103329:319782-320309 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT254932 WP_001277108.1 NZ_CP103329:319509-319775 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References