Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 51394..52037 Replicon plasmid unnamed2
Accession NZ_CP103297
Organism Escherichia coli strain 2e

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag NW892_RS23750 Protein ID WP_001044768.1
Coordinates 51621..52037 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag NW892_RS23745 Protein ID WP_001261287.1
Coordinates 51394..51624 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NW892_RS23725 (46554) 46554..47642 + 1089 WP_000952217.1 transcriptional repressor PifC -
NW892_RS23730 (47644) 47644..49869 + 2226 WP_000698737.1 P-loop NTPase fold protein -
NW892_RS23735 (49919) 49919..50818 - 900 WP_000963206.1 nucleotide-binding protein -
NW892_RS23740 (50808) 50808..51098 - 291 WP_000111771.1 hypothetical protein -
NW892_RS23745 (51394) 51394..51624 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
NW892_RS23750 (51621) 51621..52037 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
NW892_RS23755 (52199) 52199..54337 - 2139 WP_000350638.1 AAA family ATPase -
NW892_RS23760 (54691) 54691..54948 + 258 WP_000343085.1 hypothetical protein -
NW892_RS23765 (54948) 54948..55538 + 591 WP_000194575.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid fosA3 / blaTEM-1B / blaCTX-M-55 / rmtB / floR / tet(A) / aph(6)-Id - 1..64018 64018


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T254886 WP_001044768.1 NZ_CP103297:51621-52037 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT254886 WP_001261287.1 NZ_CP103297:51394-51624 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References