Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 4716033..4716833 Replicon chromosome
Accession NZ_CP103295
Organism Escherichia coli strain 2e

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag NW892_RS22720 Protein ID WP_000342449.1
Coordinates 4716033..4716560 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag NW892_RS22725 Protein ID WP_001277108.1
Coordinates 4716567..4716833 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NW892_RS22695 (4711108) 4711108..4711875 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
NW892_RS22700 (4711872) 4711872..4713149 - 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
NW892_RS22705 (4713146) 4713146..4714072 - 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
NW892_RS22710 (4714120) 4714120..4715229 - 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
NW892_RS22715 (4715653) 4715653..4716036 + 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
NW892_RS22720 (4716033) 4716033..4716560 - 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
NW892_RS22725 (4716567) 4716567..4716833 - 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
NW892_RS22730 (4716983) 4716983..4718086 - 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
NW892_RS22735 (4718358) 4718358..4719212 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
NW892_RS22740 (4719457) 4719457..4720515 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
NW892_RS22745 (4720508) 4720508..4721176 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T254875 WP_000342449.1 NZ_CP103295:c4716560-4716033 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT254875 WP_001277108.1 NZ_CP103295:c4716833-4716567 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References