Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4151301..4151955 Replicon chromosome
Accession NZ_CP103295
Organism Escherichia coli strain 2e

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag NW892_RS19875 Protein ID WP_000244777.1
Coordinates 4151301..4151708 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag NW892_RS19880 Protein ID WP_000354046.1
Coordinates 4151689..4151955 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NW892_RS19855 (4147258) 4147258..4148991 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
NW892_RS19860 (4148997) 4148997..4149707 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NW892_RS19865 (4149732) 4149732..4150628 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
NW892_RS19870 (4150740) 4150740..4151261 + 522 WP_001055874.1 flavodoxin FldB -
NW892_RS19875 (4151301) 4151301..4151708 - 408 WP_000244777.1 protein YgfX Toxin
NW892_RS19880 (4151689) 4151689..4151955 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
NW892_RS19885 (4152198) 4152198..4153178 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
NW892_RS19890 (4153255) 4153255..4153914 - 660 WP_000250274.1 hemolysin III family protein -
NW892_RS19895 (4154078) 4154078..4154389 - 312 WP_001679467.1 N(4)-acetylcytidine aminohydrolase -
NW892_RS19900 (4154434) 4154434..4155867 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
NW892_RS19905 (4155924) 4155924..4156667 - 744 Protein_3872 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T254872 WP_000244777.1 NZ_CP103295:c4151708-4151301 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT254872 WP_000354046.1 NZ_CP103295:c4151955-4151689 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References