25486

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3688183..3688857 Replicon chromosome
Accession NC_015758
Organism Mycobacterium africanum GM041182

Toxin (Protein)


Gene name vapC Uniprot ID P9WF52
Locus tag MAF_RS17345 Protein ID WP_003417282.1
Coordinates 3688183..3688611 (-) Length 143 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TI59
Locus tag MAF_RS17350 Protein ID WP_003417286.1
Coordinates 3688615..3688857 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MAF_RS17320 3684005..3684406 - 402 WP_003417264.1 cytidine deaminase -
MAF_RS17325 3684547..3684981 + 435 WP_003900017.1 succinate dehydrogenase, cytochrome b556 subunit -
MAF_RS17330 3684978..3685412 + 435 WP_003417269.1 succinate dehydrogenase hydrophobic membrane anchor subunit -
MAF_RS17335 3685541..3687313 + 1773 WP_003417273.1 succinate dehydrogenase flavoprotein subunit -
MAF_RS17340 3687313..3688104 + 792 WP_003417279.1 succinate dehydrogenase iron-sulfur subunit -
MAF_RS17345 3688183..3688611 - 429 WP_003417282.1 type II toxin-antitoxin system VapC family toxin Toxin
MAF_RS17350 3688615..3688857 - 243 WP_003417286.1 ribbon-helix-helix domain-containing protein Antitoxin
MAF_RS17355 3688979..3689593 - 615 WP_003417288.1 class I SAM-dependent methyltransferase -
MAF_RS17360 3689590..3690168 - 579 Protein_3402 molybdenum cofactor biosynthesis protein MoaE -
MAF_RS22015 3691521..3691613 - 93 Protein_3404 molybdopterin converting factor subunit 1 -
MAF_RS17375 3691614..3692147 - 534 WP_003417293.1 cyclic pyranopterin monophosphate synthase MoaC -
MAF_RS17380 3692144..3692518 - 375 WP_003417295.1 4a-hydroxytetrahydrobiopterin dehydratase -
MAF_RS17385 3692615..3693679 - 1065 WP_080549212.1 GTP 3',8-cyclase MoaA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-141)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 143 a.a.        Molecular weight: 15834.16 Da        Isoelectric Point: 8.5471

>T25486 WP_003417282.1 NC_015758:c3688611-3688183 [Mycobacterium tuberculosis variant africanum GM041182]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Download         Length: 429 bp

>T25486 NC_015758:c3688611-3688183 [Mycobacterium tuberculosis variant africanum GM041182]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8849.86 Da        Isoelectric Point: 5.1007

>AT25486 WP_003417286.1 NC_015758:c3688857-3688615 [Mycobacterium tuberculosis variant africanum GM041182]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Download         Length: 243 bp

>AT25486 NC_015758:c3688857-3688615 [Mycobacterium tuberculosis variant africanum GM041182]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBL0

Antitoxin

Source ID Structure
AlphaFold DB G0TI59

References