Detailed information of TA system TA025483

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 3155448..3155996
• Replicon: chromosome
• Accession: NC_015758
• Organism: Mycobacterium africanum GM041182

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TFG5
• Locus tag: MAF_RS14930
• Protein ID: WP_003414602.1
• Coordinates: 3155733..3155996 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: O33347
• Locus tag: MAF_RS14925
• Protein ID: WP_003414599.1
• Coordinates: 3155448..3155729 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAF_RS14900	3151071..3151928	-	858	WP_003910658.1	type I methionyl aminopeptidase	-
MAF_RS14905	3151970..3152554	-	585	WP_003414585.1	DUF1707 domain-containing protein	-
MAF_RS14910	3152712..3152906	+	195	WP_003414589.1	hypothetical protein	-
MAF_RS14915	3152903..3153283	+	381	WP_003414592.1	type II toxin-antitoxin system VapC family toxin	-
MAF_RS14920	3153365..3155176	-	1812	WP_003414596.1	penicillin-binding protein	-
MAF_RS14925	3155448..3155729	+	282	WP_003414599.1	type II toxin-antitoxin system antitoxin RelF	Antitoxin
MAF_RS14930	3155733..3155996	+	264	WP_003414602.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MAF_RS14935	3156369..3157223	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
MAF_RS14940	3157279..3158442	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
MAF_RS14945	3158459..3159673	-	1215	WP_003414610.1	zinc metalloprotease Rip	-
MAF_RS14950	3159681..3160922	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10204.82 Da
• Isoelectric Point: 11.5078

>T25483 WP_003414602.1 NC_015758:3155733-3155996 [Mycobacterium tuberculosis variant africanum GM041182]
VPYTVRFTTTARRDLHKLPPRILAAVVEFAFGDLSREPLRVGKPLRRELAGTFSARRGTYRLLYRIDDEHTTVVILRVDH
RADIYRR

Nucleotide

• Length: 264 bp

>T25483 NC_015758:3155733-3155996 [Mycobacterium tuberculosis variant africanum GM041182]
GTGCCTTACACCGTGCGGTTCACCACAACCGCGCGTCGAGACCTCCACAAGCTGCCACCGCGAATCCTCGCGGCAGTGGT
CGAATTCGCGTTCGGCGATCTGTCGCGCGAGCCCCTGCGGGTGGGCAAGCCCCTTCGGCGCGAGTTGGCCGGCACGTTCA
GCGCGCGTCGCGGAACGTACCGCCTGCTGTACCGGATTGACGACGAGCACACAACGGTAGTGATCCTGCGCGTCGATCAC
CGCGCGGACATCTACCGCCGATAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10183.40 Da
• Isoelectric Point: 4.3440

>AT25483 WP_003414599.1 NC_015758:3155448-3155729 [Mycobacterium tuberculosis variant africanum GM041182]
MRILPISTIKGKLNEFVDAVSSTQDQITITKNGAPAAVLVGADEWESLQETLYWLAQPGIRESIAEADADIASGRTYGED
EIRAEFGVPRRPH

Nucleotide

• Length: 282 bp

>AT25483 NC_015758:3155448-3155729 [Mycobacterium tuberculosis variant africanum GM041182]
ATGCGGATACTGCCGATTTCGACGATCAAGGGCAAGCTCAATGAGTTCGTCGACGCGGTCTCGTCGACACAGGACCAGAT
CACCATCACCAAGAACGGTGCACCCGCAGCCGTTCTGGTCGGCGCCGACGAGTGGGAATCGTTGCAGGAGACGCTGTACT
GGCTGGCGCAACCCGGAATCAGGGAGTCGATCGCTGAAGCCGACGCCGACATTGCCTCCGGCCGCACCTACGGCGAAGAC
GAGATCCGCGCCGAATTCGGCGTCCCGCGACGCCCCCACTGA

Structures

Toxin

• PDB: 3G5O

Antitoxin

• AlphaFold DB: A0A7U4BWP8