Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 518331..518967 Replicon chromosome
Accession NZ_CP103066
Organism Bacillus subtilis strain 0137

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag NXS96_RS02640 Protein ID WP_003156187.1
Coordinates 518617..518967 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag NXS96_RS02635 Protein ID WP_003225183.1
Coordinates 518331..518612 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NXS96_RS02615 (514690) 514690..515289 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
NXS96_RS02620 (515384) 515384..515749 + 366 WP_003234281.1 holo-ACP synthase -
NXS96_RS02625 (515915) 515915..516931 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
NXS96_RS02630 (517046) 517046..518215 + 1170 WP_003234284.1 alanine racemase -
NXS96_RS02635 (518331) 518331..518612 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
NXS96_RS02640 (518617) 518617..518967 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NXS96_RS02645 (519082) 519082..519906 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
NXS96_RS02650 (519911) 519911..520276 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
NXS96_RS02655 (520280) 520280..520681 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
NXS96_RS02660 (520693) 520693..521700 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
NXS96_RS02665 (521762) 521762..522091 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
NXS96_RS02670 (522088) 522088..522570 + 483 WP_003234299.1 anti-sigma B factor RsbW -
NXS96_RS02675 (522536) 522536..523324 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
NXS96_RS02680 (523324) 523324..523923 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T254828 WP_003156187.1 NZ_CP103066:518617-518967 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT254828 WP_003225183.1 NZ_CP103066:518331-518612 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References