Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4618142..4618952 Replicon chromosome
Accession NZ_CP102993
Organism Klebsiella pneumoniae strain KPA3

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag NW255_RS22690 Protein ID WP_004178461.1
Coordinates 4618142..4618675 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag NW255_RS22695 Protein ID WP_002887278.1
Coordinates 4618686..4618952 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NW255_RS22685 (4616973) 4616973..4618094 + 1122 WP_002887282.1 cupin domain-containing protein -
NW255_RS22690 (4618142) 4618142..4618675 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
NW255_RS22695 (4618686) 4618686..4618952 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
NW255_RS22700 (4619055) 4619055..4620488 - 1434 WP_258825656.1 Cu(+)/Ag(+) sensor histidine kinase -
NW255_RS22705 (4620478) 4620478..4621161 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
NW255_RS22710 (4621333) 4621333..4622718 + 1386 WP_004178457.1 efflux transporter outer membrane subunit -
NW255_RS22715 (4622736) 4622736..4623080 + 345 WP_004197273.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T254820 WP_004178461.1 NZ_CP102993:c4618675-4618142 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT254820 WP_002887278.1 NZ_CP102993:c4618952-4618686 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References