Detailed information of TA system TA025482

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3114531..3115135
• Replicon: chromosome
• Accession: NC_015758
• Organism: Mycobacterium africanum GM041182

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: MAF_RS14730
• Protein ID: WP_003414492.1
• Coordinates: 3114531..3114923 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MAF_RS14735
• Protein ID: WP_003910640.1
• Coordinates: 3114920..3115135 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAF_RS14700	3109681..3110469	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
MAF_RS14705	3110803..3111348	-	546	WP_031667139.1	DUF1802 family protein	-
MAF_RS14710	3111620..3112504	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
MAF_RS14715	3112507..3113394	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
MAF_RS14720	3113699..3114244	-	546	WP_013988952.1	DUF1802 family protein	-
MAF_RS14725	3114241..3114510	-	270	WP_003414489.1	DUF2277 family protein	-
MAF_RS14730	3114531..3114923	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
MAF_RS14735	3114920..3115135	-	216	WP_003910640.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MAF_RS14740	3115182..3115931	+	750	WP_003414497.1	enoyl-CoA hydratase	-
MAF_RS14745	3116010..3117092	-	1083	WP_003414499.1	ABC transporter ATP-binding protein	-
MAF_RS14750	3117085..3118395	-	1311	WP_013988953.1	ABC transporter substrate-binding protein	-
MAF_RS14755	3118398..3119225	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
MAF_RS14760	3119222..3120133	-	912	WP_013988954.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T25482 WP_003414492.1 NC_015758:c3114923-3114531 [Mycobacterium tuberculosis variant africanum GM041182]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T25482 NC_015758:c3114923-3114531 [Mycobacterium tuberculosis variant africanum GM041182]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7495.55 Da
• Isoelectric Point: 4.9732

>AT25482 WP_003910640.1 NC_015758:c3115135-3114920 [Mycobacterium tuberculosis variant africanum GM041182]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVAWNVS

Nucleotide

• Length: 216 bp

>AT25482 NC_015758:c3115135-3114920 [Mycobacterium tuberculosis variant africanum GM041182]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTGCGTGGAACGTTTCATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8