Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5204380..5205005 Replicon chromosome
Accession NZ_CP102987
Organism Klebsiella pneumoniae strain KPA2

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NW253_RS25435 Protein ID WP_002882817.1
Coordinates 5204380..5204763 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NW253_RS25440 Protein ID WP_004150355.1
Coordinates 5204763..5205005 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NW253_RS25420 (5201746) 5201746..5202648 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NW253_RS25425 (5202645) 5202645..5203280 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NW253_RS25430 (5203277) 5203277..5204206 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NW253_RS25435 (5204380) 5204380..5204763 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NW253_RS25440 (5204763) 5204763..5205005 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NW253_RS25445 (5205210) 5205210..5206127 + 918 WP_021466676.1 alpha/beta hydrolase -
NW253_RS25450 (5206141) 5206141..5207082 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NW253_RS25455 (5207127) 5207127..5207564 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NW253_RS25460 (5207561) 5207561..5208421 - 861 WP_004146232.1 virulence factor BrkB family protein -
NW253_RS25465 (5208415) 5208415..5209014 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T254805 WP_002882817.1 NZ_CP102987:c5204763-5204380 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT254805 WP_004150355.1 NZ_CP102987:c5205005-5204763 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References