Detailed information of TA system TA025480

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3086734..3087304
• Replicon: chromosome
• Accession: NC_015758
• Organism: Mycobacterium africanum GM041182

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: MAF_RS14580
• Protein ID: WP_003414166.1
• Coordinates: 3086734..3087090 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: MAF_RS14585
• Protein ID: WP_003901465.1
• Coordinates: 3087074..3087304 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAF_RS14560	3082186..3083874	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
MAF_RS14565	3083878..3084204	-	327	WP_003414157.1	hypothetical protein	-
MAF_RS14570	3084377..3084964	+	588	WP_044501731.1	DUF3558 family protein	-
MAF_RS14575	3084983..3086632	+	1650	WP_003899485.1	CocE/NonD family hydrolase	-
MAF_RS14580	3086734..3087090	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
MAF_RS14585	3087074..3087304	-	231	WP_003901465.1	antitoxin MazE	Antitoxin
MAF_RS14590	3087347..3088390	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
MAF_RS21960	3088581..3088856	+	276	WP_003911993.1	DUF1778 domain-containing protein	-
MAF_RS21965	3089032..3089286	-	255	WP_003917684.1	hypothetical protein	-
MAF_RS14605	3089434..3089838	+	405	WP_003414181.1	hypothetical protein	-
MAF_RS14610	3089835..3090026	+	192	WP_003414184.1	hypothetical protein	-
MAF_RS14615	3090090..3091379	+	1290	Protein_2865	transposase family protein	-
MAF_RS14620	3091613..3091870	+	258	WP_003899489.1	hypothetical protein	-
MAF_RS14625	3091975..3092286	+	312	WP_003414190.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T25480 WP_003414166.1 NC_015758:c3087090-3086734 [Mycobacterium tuberculosis variant africanum GM041182]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T25480 NC_015758:c3087090-3086734 [Mycobacterium tuberculosis variant africanum GM041182]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT25480 WP_003901465.1 NC_015758:c3087304-3087074 [Mycobacterium tuberculosis variant africanum GM041182]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT25480 NC_015758:c3087304-3087074 [Mycobacterium tuberculosis variant africanum GM041182]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• PDB: 7DU5
• PDB: 6KYT
• AlphaFold DB: A0A7U4FBW3