Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1435118..1435854 Replicon chromosome
Accession NZ_CP102987
Organism Klebsiella pneumoniae strain KPA2

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag NW253_RS07040 Protein ID WP_032433360.1
Coordinates 1435372..1435854 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag NW253_RS07035 Protein ID WP_003026799.1
Coordinates 1435118..1435384 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NW253_RS07010 (1430764) 1430764..1431903 + 1140 WP_032433368.1 mannitol dehydrogenase -
NW253_RS07015 (1431932) 1431932..1432594 + 663 WP_032433366.1 dihydroxyacetone kinase subunit DhaL -
NW253_RS07020 (1432578) 1432578..1433582 + 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
NW253_RS07025 (1433600) 1433600..1434232 + 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
NW253_RS07030 (1434242) 1434242..1434805 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
NW253_RS07035 (1435118) 1435118..1435384 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
NW253_RS07040 (1435372) 1435372..1435854 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
NW253_RS07045 (1436054) 1436054..1437457 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
NW253_RS07050 (1437486) 1437486..1438118 - 633 WP_001567369.1 hypothetical protein -
NW253_RS07055 (1438498) 1438498..1439097 - 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
NW253_RS07060 (1439310) 1439310..1440254 - 945 WP_077254249.1 fimbrial protein -
NW253_RS07065 (1440266) 1440266..1440844 - 579 WP_032432061.1 type 1 fimbrial protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1403063..1450145 47082
- flank IS/Tn - - 1436054..1437457 1403


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T254795 WP_032433360.1 NZ_CP102987:1435372-1435854 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT254795 WP_003026799.1 NZ_CP102987:1435118-1435384 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References