Detailed information of TA system TA025478

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3046738..3047417
• Replicon: chromosome
• Accession: NC_015758
• Organism: Mycobacterium africanum GM041182

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: MAF_RS14355
• Protein ID: WP_003414059.1
• Coordinates: 3046738..3047154 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: MAF_RS14360
• Protein ID: WP_003414061.1
• Coordinates: 3047151..3047417 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAF_RS14335	3042790..3043692	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
MAF_RS14340	3043761..3044513	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
MAF_RS14345	3044757..3045032	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
MAF_RS14350	3045029..3046651	-	1623	WP_013988948.1	type I restriction-modification system subunit M	-
MAF_RS14355	3046738..3047154	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
MAF_RS14360	3047151..3047417	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MAF_RS14365	3047443..3047838	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
MAF_RS14370	3047835..3048104	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
MAF_RS14375	3048114..3049208	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
MAF_RS14380	3049205..3049624	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
MAF_RS14385	3049698..3050177	-	480	WP_003414073.1	dihydrofolate reductase	-
MAF_RS14390	3050248..3051048	-	801	WP_003911953.1	thymidylate synthase	-
MAF_RS14395	3051204..3051941	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T25478 WP_003414059.1 NC_015758:c3047154-3046738 [Mycobacterium tuberculosis variant africanum GM041182]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T25478 NC_015758:c3047154-3046738 [Mycobacterium tuberculosis variant africanum GM041182]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT25478 WP_003414061.1 NC_015758:c3047417-3047151 [Mycobacterium tuberculosis variant africanum GM041182]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT25478 NC_015758:c3047417-3047151 [Mycobacterium tuberculosis variant africanum GM041182]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64