25476

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2857168..2857805 Replicon chromosome
Accession NC_015758
Organism Mycobacterium africanum GM041182

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag MAF_RS13370 Protein ID WP_003413180.1
Coordinates 2857168..2857563 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag MAF_RS13375 Protein ID WP_003413183.1
Coordinates 2857560..2857805 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MAF_RS13330 2853253..2853912 + 660 WP_003900846.1 LppA family lipoprotein -
MAF_RS13335 2853909..2854568 + 660 WP_003900847.1 hypothetical protein -
MAF_RS13340 2854565..2855227 + 663 WP_003900848.1 hypothetical protein -
MAF_RS13345 2855224..2855502 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
MAF_RS13350 2855595..2856008 + 414 WP_003413164.1 PIN domain nuclease -
MAF_RS13355 2856047..2856304 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
MAF_RS13360 2856301..2856678 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
MAF_RS13365 2856694..2857068 - 375 WP_003413177.1 hypothetical protein -
MAF_RS13370 2857168..2857563 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
MAF_RS13375 2857560..2857805 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
MAF_RS13380 2858216..2858635 - 420 WP_003413190.1 A24 family peptidase -
MAF_RS13385 2858647..2859456 - 810 WP_003910591.1 shikimate dehydrogenase -
MAF_RS13390 2859453..2860706 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
MAF_RS13395 2860699..2861211 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T25476 WP_003413180.1 NC_015758:c2857563-2857168 [Mycobacterium tuberculosis variant africanum GM041182]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T25476 NC_015758:c2857563-2857168 [Mycobacterium tuberculosis variant africanum GM041182]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT25476 WP_003413183.1 NC_015758:c2857805-2857560 [Mycobacterium tuberculosis variant africanum GM041182]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT25476 NC_015758:c2857805-2857560 [Mycobacterium tuberculosis variant africanum GM041182]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References