25475

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2856047..2856678 Replicon chromosome
Accession NC_015758
Organism Mycobacterium africanum GM041182

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag MAF_RS13360 Protein ID WP_003413174.1
Coordinates 2856301..2856678 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag MAF_RS13355 Protein ID WP_003413167.1
Coordinates 2856047..2856304 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MAF_RS13320 2851305..2851619 + 315 WP_044501729.1 hypothetical protein -
MAF_RS13325 2851915..2853126 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
MAF_RS13330 2853253..2853912 + 660 WP_003900846.1 LppA family lipoprotein -
MAF_RS13335 2853909..2854568 + 660 WP_003900847.1 hypothetical protein -
MAF_RS13340 2854565..2855227 + 663 WP_003900848.1 hypothetical protein -
MAF_RS13345 2855224..2855502 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
MAF_RS13350 2855595..2856008 + 414 WP_003413164.1 PIN domain nuclease -
MAF_RS13355 2856047..2856304 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein Antitoxin
MAF_RS13360 2856301..2856678 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
MAF_RS13365 2856694..2857068 - 375 WP_003413177.1 hypothetical protein -
MAF_RS13370 2857168..2857563 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
MAF_RS13375 2857560..2857805 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
MAF_RS13380 2858216..2858635 - 420 WP_003413190.1 A24 family peptidase -
MAF_RS13385 2858647..2859456 - 810 WP_003910591.1 shikimate dehydrogenase -
MAF_RS13390 2859453..2860706 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
MAF_RS13395 2860699..2861211 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T25475 WP_003413174.1 NC_015758:2856301-2856678 [Mycobacterium tuberculosis variant africanum GM041182]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T25475 NC_015758:2856301-2856678 [Mycobacterium tuberculosis variant africanum GM041182]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT25475 WP_003413167.1 NC_015758:2856047-2856304 [Mycobacterium tuberculosis variant africanum GM041182]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT25475 NC_015758:2856047-2856304 [Mycobacterium tuberculosis variant africanum GM041182]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References