Detailed information of TA system TA025473

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2841052..2841692
• Replicon: chromosome
• Accession: NC_015758
• Organism: Mycobacterium africanum GM041182

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: MAF_RS13260
• Protein ID: WP_003412970.1
• Coordinates: 2841052..2841471 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MAF_RS13265
• Protein ID: WP_003910588.1
• Coordinates: 2841468..2841692 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAF_RS13235	2836640..2837362	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
MAF_RS13240	2837879..2838106	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
MAF_RS13245	2838103..2838504	+	402	WP_003412963.1	PIN domain-containing protein	-
MAF_RS13250	2838539..2839459	-	921	WP_003412965.1	restriction endonuclease	-
MAF_RS22160	2839800..2840045	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
MAF_RS13255	2840104..2841051	+	948	WP_162097749.1	Lsr2 family protein	-
MAF_RS13260	2841052..2841471	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
MAF_RS13265	2841468..2841692	-	225	WP_003910588.1	hypothetical protein	Antitoxin
MAF_RS13270	2841723..2844566	-	2844	WP_003910589.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
MAF_RS13275	2844638..2845039	-	402	WP_003412981.1	hypothetical protein	-
MAF_RS13280	2845039..2845509	-	471	WP_003412985.1	transcription antitermination factor NusB	-
MAF_RS13285	2845512..2846075	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T25473 WP_003412970.1 NC_015758:c2841471-2841052 [Mycobacterium tuberculosis variant africanum GM041182]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T25473 NC_015758:c2841471-2841052 [Mycobacterium tuberculosis variant africanum GM041182]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8021.99 Da
• Isoelectric Point: 4.0057

>AT25473 WP_003910588.1 NC_015758:c2841692-2841468 [Mycobacterium tuberculosis variant africanum GM041182]
MRTTLQIDDDVLEDVRSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT25473 NC_015758:c2841692-2841468 [Mycobacterium tuberculosis variant africanum GM041182]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGTTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08