Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2222121..2222707 Replicon chromosome
Accession NC_015758
Organism Mycobacterium africanum GM041182

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag MAF_RS10365 Protein ID WP_003410010.1
Coordinates 2222121..2222465 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag MAF_RS10370 Protein ID WP_003410014.1
Coordinates 2222459..2222707 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MAF_RS10330 2217827..2218426 + 600 WP_003409989.1 L-lysine exporter -
MAF_RS10335 2218842..2219270 + 429 WP_003409992.1 cellulose-binding protein -
MAF_RS10340 2219496..2220035 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
MAF_RS10345 2220555..2221115 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
MAF_RS10350 2221112..2221453 - 342 WP_003410003.1 DUF2384 domain-containing protein -
MAF_RS10355 2221539..2221796 + 258 WP_003410006.1 hypothetical protein -
MAF_RS10360 2221697..2222032 - 336 WP_003410009.1 dehydrogenase -
MAF_RS10365 2222121..2222465 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
MAF_RS10370 2222459..2222707 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
MAF_RS10375 2222807..2225122 - 2316 WP_003899120.1 cation transporter ATPase CptG -
MAF_RS10380 2225119..2225391 - 273 WP_003410017.1 DUF1490 family protein -
MAF_RS10385 2225444..2225800 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
MAF_RS10390 2225957..2226717 + 761 Protein_2039 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T25466 WP_003410010.1 NC_015758:c2222465-2222121 [Mycobacterium tuberculosis variant africanum GM041182]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T25466 NC_015758:c2222465-2222121 [Mycobacterium tuberculosis variant africanum GM041182]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT25466 WP_003410014.1 NC_015758:c2222707-2222459 [Mycobacterium tuberculosis variant africanum GM041182]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT25466 NC_015758:c2222707-2222459 [Mycobacterium tuberculosis variant africanum GM041182]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References