Detailed information of TA system TA025464

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2213229..2213917
• Replicon: chromosome
• Accession: NC_015758
• Organism: Mycobacterium africanum GM041182

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P0A653
• Locus tag: MAF_RS10300
• Protein ID: WP_003409958.1
• Coordinates: 2213229..2213648 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ28
• Locus tag: MAF_RS10305
• Protein ID: WP_003409968.1
• Coordinates: 2213657..2213917 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAF_RS10275	2208235..2208540	+	306	Protein_2016	ABC transporter permease	-
MAF_RS10280	2208724..2209572	+	849	WP_003910485.1	class I SAM-dependent methyltransferase	-
MAF_RS10285	2209535..2210980	-	1446	WP_003409946.1	APC family permease	-
MAF_RS10290	2211159..2211845	-	687	WP_003409954.1	immunoprotective protein Mpt64	-
MAF_RS10295	2212036..2213004	-	969	WP_003409956.1	class 1b ribonucleoside-diphosphate reductase subunit beta	-
MAF_RS10300	2213229..2213648	-	420	WP_003409958.1	type II toxin-antitoxin system VapC family toxin	Toxin
MAF_RS10305	2213657..2213917	-	261	WP_003409968.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MAF_RS10310	2214060..2215736	+	1677	WP_003899118.1	PecA family PE domain-processing aspartic protease	-
MAF_RS10315	2215724..2216377	-	654	WP_003409976.1	cutinase Cfp21	-
MAF_RS10320	2216492..2216722	+	231	WP_003409980.1	DUF167 domain-containing protein	-
MAF_RS10325	2216807..2217718	-	912	WP_003409986.1	ArgP/LysG family DNA-binding transcriptional regulator	-
MAF_RS10330	2217827..2218426	+	600	WP_003409989.1	L-lysine exporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Antitoxin VapB-like (2-81)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14724.89 Da
• Isoelectric Point: 7.9535

>T25464 WP_003409958.1 NC_015758:c2213648-2213229 [Mycobacterium tuberculosis variant africanum GM041182]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDLEFRGVVGYWPGVA

Nucleotide

• Length: 420 bp

>T25464 NC_015758:c2213648-2213229 [Mycobacterium tuberculosis variant africanum GM041182]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9528.88 Da
• Isoelectric Point: 5.1007

>AT25464 WP_003409968.1 NC_015758:c2213917-2213657 [Mycobacterium tuberculosis variant africanum GM041182]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Nucleotide

• Length: 261 bp

>AT25464 NC_015758:c2213917-2213657 [Mycobacterium tuberculosis variant africanum GM041182]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Structures

Toxin

• AlphaFold DB: A0A829C4H9

Antitoxin

• AlphaFold DB: A0A829C483