Detailed information of TA system TA025461

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2195178..2195881
• Replicon: chromosome
• Accession: NC_015758
• Organism: Mycobacterium africanum GM041182

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: MAF_RS10150
• Protein ID: WP_003409778.1
• Coordinates: 2195178..2195507 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: MAF_RS21105
• Protein ID: WP_003409780.1
• Coordinates: 2195504..2195881 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAF_RS10130	2191561..2192631	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
MAF_RS10135	2192628..2193143	+	516	WP_003409718.1	flavin reductase family protein	-
MAF_RS10140	2193140..2194201	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
MAF_RS10145	2194198..2194968	+	771	WP_003409775.1	SDR family oxidoreductase	-
MAF_RS10150	2195178..2195507	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MAF_RS21105	2195504..2195881	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
MAF_RS10160	2195878..2196468	-	591	WP_003910477.1	SEC-C domain-containing protein	-
MAF_RS10165	2196523..2197887	+	1365	WP_013988890.1	HNH endonuclease	-
MAF_RS10170	2198042..2198494	-	453	WP_003899095.1	lipoprotein	-
MAF_RS10175	2198558..2198959	+	402	WP_003409869.1	hypothetical protein	-
MAF_RS10180	2198952..2199134	-	183	WP_003409870.1	hypothetical protein	-
MAF_RS10185	2199248..2199598	-	351	WP_003910480.1	hypothetical protein	-
MAF_RS10190	2199609..2200511	-	903	WP_003409874.1	hypothetical protein	-
MAF_RS10195	2200532..2200723	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T25461 WP_003409778.1 NC_015758:c2195507-2195178 [Mycobacterium tuberculosis variant africanum GM041182]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T25461 NC_015758:c2195507-2195178 [Mycobacterium tuberculosis variant africanum GM041182]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT25461 WP_003409780.1 NC_015758:c2195881-2195504 [Mycobacterium tuberculosis variant africanum GM041182]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT25461 NC_015758:c2195881-2195504 [Mycobacterium tuberculosis variant africanum GM041182]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8