Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1939373..1939986 Replicon chromosome
Accession NC_015758
Organism Mycobacterium africanum GM041182

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag MAF_RS09025 Protein ID WP_003408465.1
Coordinates 1939373..1939762 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag MAF_RS09030 Protein ID WP_003408469.1
Coordinates 1939759..1939986 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MAF_RS08995 1935002..1935916 + 915 WP_003898985.1 3-hydroxyacyl-CoA dehydrogenase family protein -
MAF_RS09000 1935919..1936749 + 831 WP_003408448.1 cyclase family protein -
MAF_RS09005 1936749..1937099 + 351 WP_003898986.1 cupin domain-containing protein -
MAF_RS09010 1937152..1937970 + 819 WP_003408456.1 3-keto-5-aminohexanoate cleavage protein -
MAF_RS09015 1937984..1938763 + 780 WP_003408460.1 IclR family transcriptional regulator -
MAF_RS09025 1939373..1939762 - 390 WP_003408465.1 PIN domain-containing protein Toxin
MAF_RS09030 1939759..1939986 - 228 WP_003408469.1 antitoxin Antitoxin
MAF_RS09035 1940204..1941688 + 1485 WP_003408473.1 carboxylase -
MAF_RS09040 1941685..1942932 + 1248 WP_003408476.1 serine hydrolase -
MAF_RS09045 1942975..1943394 - 420 WP_003408483.1 hypothetical protein -
MAF_RS09050 1943384..1944094 - 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T25459 WP_003408465.1 NC_015758:c1939762-1939373 [Mycobacterium tuberculosis variant africanum GM041182]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T25459 NC_015758:c1939762-1939373 [Mycobacterium tuberculosis variant africanum GM041182]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT25459 WP_003408469.1 NC_015758:c1939986-1939759 [Mycobacterium tuberculosis variant africanum GM041182]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT25459 NC_015758:c1939986-1939759 [Mycobacterium tuberculosis variant africanum GM041182]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9

Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References