25457

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1689898..1690514 Replicon chromosome
Accession NC_015758
Organism Mycobacterium africanum GM041182

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag MAF_RS07935 Protein ID WP_003407593.1
Coordinates 1690197..1690514 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A109SWY9
Locus tag MAF_RS07930 Protein ID WP_003910377.1
Coordinates 1689898..1690200 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MAF_RS07920 1685784..1687631 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
MAF_RS07925 1687632..1689884 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
MAF_RS07930 1689898..1690200 + 303 WP_003910377.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
MAF_RS07935 1690197..1690514 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
MAF_RS07940 1690511..1691515 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
MAF_RS07945 1691568..1692857 + 1290 WP_013988847.1 serine hydrolase -
MAF_RS07950 1692930..1693703 - 774 WP_003912632.1 class I SAM-dependent methyltransferase -
MAF_RS07955 1693761..1693952 - 192 WP_003407609.1 dodecin family protein -
MAF_RS22125 1693983..1694207 - 225 WP_160522526.1 NUDIX domain-containing protein -
MAF_RS07965 1694477..1695505 + 1029 WP_013988849.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T25457 WP_003407593.1 NC_015758:1690197-1690514 [Mycobacterium tuberculosis variant africanum GM041182]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T25457 NC_015758:1690197-1690514 [Mycobacterium tuberculosis variant africanum GM041182]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11203.45 Da        Isoelectric Point: 8.5236

>AT25457 WP_003910377.1 NC_015758:1689898-1690200 [Mycobacterium tuberculosis variant africanum GM041182]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARAWRNARQQRSAQ

Download         Length: 303 bp

>AT25457 NC_015758:1689898-1690200 [Mycobacterium tuberculosis variant africanum GM041182]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCTGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
AlphaFold DB A0A109SWY9

References